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Biotin antibody (FITC)

The Goat anti-Biotin antibody has been validated for ELISA, FACS, IF and IM. It is suitable to detect Biotin in samples from .
Catalog No. ABIN965471

Quick Overview for Biotin antibody (FITC) (ABIN965471)

Target

Biotin

Reactivity

Please inquire

Host

  • 63
  • 42
  • 41
Goat

Conjugate

  • 40
  • 13
  • 9
  • 8
  • 8
  • 6
  • 5
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
This Biotin antibody is conjugated to FITC

Application

  • 82
  • 61
  • 56
  • 50
  • 38
  • 33
  • 16
  • 13
  • 11
  • 10
  • 9
  • 8
  • 8
  • 6
  • 4
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunomicroscopy (IM)
  • Specificity

    This antibody reacts with Biotin

    Cross-Reactivity (Details)

    This antibody does not react with free BSA or free KLH

    Characteristics

    Concentration: 1.5 mg/ml (E 1% at 280 nm = 13.0)
    Conjugate: Fluorescein-5-isothiocyanate (FITC) Amax = 494 nm, Emax = 518 nm

    Purification

    Affinity purified using solid phase biotin

    Purity

    > 95 % based on SDS-PAGE

    Immunogen

    BSA-Biotin, KLH-Biotin
  • Application Notes

    This Conjugate is suitable for fluorescent immunomicroscopy and flow cytometry. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:20 - 1:2,000 for most applications

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1.5 mg/mL

    Buffer

    10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Prepare working dilution prior to use and then discard.

    Storage

    4 °C

    Storage Comment

    2-8 °C
  • Target

    Biotin

    Target Type

    Chemical
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